DNA Sequencing
DNA sequencing is a fundamental tool for describing genetic information of organisms. Among the most widely used approaches are Whole-genome sequencing (WGS), which analyzes the entire genome of an organism, and Amplicon Sequencing, focused on specific regions, such as ITS (internal transcribed spacer) and 16S, widely applied in the identification of microorganisms.
- Whole-genome sequencing (WGS): Allows comprehensive analysis of the genome, including coding and non-coding regions. This approach provides a detailed view of the genetic composition and is ideal for complex studies, such as evolution, genomic mutations, and discovery of new genes.
- Targeted Sequencing (ITS, 16S): Focused on specific regions of DNA, such as the ITS, used to identify fungi, and the 16S rRNA gene, widely used in the identification of bacteria. This technique is more economical and efficient for taxonomic analyses or studies of microbial communities.
- Total Metagenomics (Shotgun Metagenomics): Without the amplification of specific regions, this technique involves the sequencing of all DNA present in an environmental sample. This allows the analysis of all genetic material in the sample, including bacteria, archaea, viruses, fungi, and other eukaryotes, without amplification bias.
Both techniques are complementary methodological alternatives in solutions in environmental biotechnology, agriculture, and other areas, contributing to the identification of microorganisms, the production of biomolecules, biodiversity studies and sustainable energy solutions.
Available services*
- Whole genome sequencing (WGS):
- Platforms: Illumina short reads and long-reads (Oxford Nanopore Technologies)
- Service: Quality analysis, DNA library preparation, DNA sequencing.
- Metagenomics – 16S amplicon sequencing, ITS:
- Service: Quality analysis, 16S and ITS amplicon library preparation
- Total metagenomics – shotgun metagenomics:
- Service: Quality analysis, total DNA library preparation
*Outsourced Services
Applications
- Whole-genome sequencing (WGS): This approach provides a detailed view of the genetic composition and is ideal for complex studies, such as evolution, genomic mutations, and discovery of new genes.
- Targeted Sequencing (ITS, 16S): This technique is more economical and efficient for taxonomic analyses or studies of microbial communities.
- Total Metagenomics (Shotgun Metagenomics): This allows the analysis of all genetic material in the sample, including bacteria, archaea, viruses, fungi, and other eukaryotes, without amplification bias.
Sample requirements
For whole genome sequencing and amplicons, high-quality genomic DNA and purified samples suitable for 16S and ITS amplification must be submitted. Please contact us for detailed guidance on sample collection and submission.
Contact us
Have a specific project in mind? Send us a message and our team will be happy to discuss your needs. We are ready to collaborate on customized solutions for your research.